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KMID : 0350519930460041667
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1667 ~ p.1681
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Establishment and Characterization of a Cell Line Derived from a Squamous Cell Carcinoma of the Human Uterine Cevix
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Abstract
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An established cell line derived from a primary squamous cell carcinoma of the uterine cervix in a 32 year old patient was investigated for its morphology, ultrastructure, genetic signature analysis, karyotype, hormone and protein secretion,
tumor
markers, and tumorigenesis in the nude mice.
The histology of the original tumor was large, non-keratinizing squamous cell carcinoma and was compared with that occurring in the nude mouse after the cultured cells were inoculated.
These cells were continously propagated in vitro during the past 46 months and grown in a monolayered sheet with a doubling time of about 35 hours.
When cells from passage 30 or 90 were injected subcutaneously into nede mice, tumors developed.
The histologic pattrn of tumor grown from cervical carcinoma cells and that of the biopsy of the original tumor of the uterine cervix were indistinguishable. The cells were highly tumorigenic in nude mice.
The epithelial nature of the xenografts, like the original tumor specimen, was also confirmed by transmission electron microscopy, which demonstrated the presence of desmosomes and tonofilaments.
Repeated chromosome analysis reveled a stable clone with the modal chromosome number 80. The metaphase of this cell line has 7 kinds of structural chromosomal anomalies (M1-M7).
No Hela marker chrmosomes were identified.
Isoenzyme analysis of glucose 6-phosphate dehydrogenase showed type B ixozyme pattern.
This cell line produced human chorionic gonadotropin, estrogen, and TA-4.
At present, these cells have undergone 350 passages and post nude mouse culture cells have undergone 370 passages.
These characteristics make this cervical carcinoma cell line a suitable experimental model for the treatment of human cervical carcinoma and for the biologic studies of human solid tumors.
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KEYWORD
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